|Ghosh, Ritesh - Yeungnam University|
|Choi, Bosung - Yeungnam University|
|Cho, Byoung-kwan - Chungnam National University|
|Lim, Hyoun-sub - Chungnam National University|
|Park, Sang Un - Chungnam National University|
|Bae, Hyeun-jong - Chonnam National University|
|Natarajan, Savithiry - Savi|
|Bae, Hanhong - Yeungnam University|
Submitted to: The Scientific World
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/29/2013
Publication Date: 2/26/2014
Citation: Ghosh, R., Choi, B., Cho, B., Lim, H., Park, S., Bae, H., Natarajan, S.S., Bae, H. 2014. Characterization of developmental and stress mediated expression of cinnamoyl-CoA reductase (CCR) in kenaf (Hibiscus cannabinus L.). The Scientific World. DOI: 10.1155/2014/601845.
Interpretive Summary: Kenaf (Hisbiscus cannabinus) is an annual rapidly growing plant and is used for making rope, cordage, canvas, sacking, carpet backing, nets, table cloths etc. It has recently gained more attention as a possible source for the production of biomass fuels. Plant derived biofuel production is faced with the technical challenge within biological conversion due to higher interactions between lignin and polysaccharides. To address this issue, the study of the regulation of genes involved in the metabolic pathways leading to lignin production is important. We examined the changes in gene activity associated with one of the pathway genes called “Cinnamoyl-CoA reductase (CCR)” in different stress treatments using various Kenaf plant tissues. We found that the CCR gene was more highly expressed in the flower due to drought and NaCl treatments. This information will be useful for scientists in selecting suitable plant genotypes for biomass production.
Technical Abstract: Cinnamoyl-CoA reductase (CCR) is an important enzyme for lignin biosynthesis as it catalyzes the first specific committed step in monolignol biosynthesis. We have cloned a full length coding sequence of CCR from kenaf (Hibiscus cannabinus L.), which contains a 1,020-bp open reading frame (ORF), encoding 339 amino acids of 37.37 kDa, with an isoelectric point (pI) of 6.27 (JX524276, HcCCR2). BLAST result found that it has high homology with other plant CCR orthologs. Multiple alignment with other plant CCR sequences showed that it contains two highly conserved motifs: NAD (P)-binding domain (VTGAGGFIASWMVKLLLEKGY) at N-terminal, and probable catalytic domain (NWYCYGK). According to phylogenetic analysis, it was closely related to CCR sequences of Gossypium hirsutum (ACQ59094) and Populus trichocarpa (CAC07424). HcCCR2 showed ubiquitous expression in various kenaf tissues and the highest expression was detected in mature flower. HcCCR2 was expressed differentially in response to various stresses, and the highest expression was observed by drought and NaCl treatments.