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Title: Complete nucleotide sequence of Rose yellow leaf virus, a new member of the family Tombusviridae

item Mollov, Dimitre
item LOCKHART, BEN - University Of Minnesota
item ZLESAK, DAVID - University Of Wisconsin

Submitted to: Archives of Virology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/29/2014
Publication Date: 5/20/2014
Citation: Mollov, D.S., Lockhart, B., Zlesak, D. 2014. Complete nucleotide sequence of Rose yellow leaf virus, a new member of the family Tombusviridae. Archives of Virology. Arch. Virol. DOI 10.1007/s00705-014-2106-3.

Interpretive Summary: Rose (Rosa spp.) is one of the most utilized plants worldwide both in cut flowers and landscapes. A number of viral diseases including Rose yellow leaf virus (RYLV), a previously uncharacterized virus that causes leaf yellowing and premature plant senescence, affect roses and reduce plants aesthetic and market values. It is important to detect and eliminate virus infected material prior to propagation and shipment. In this report the RYLV is described and its genomic sequence is determined for the first time. The data presented in this study provides information for the proper classification of RYLV. The completion of the genome sequence facilitates reliable detection methods that can be utilized to screen rose genetic material and develop better control measures in the trade and production industries.

Technical Abstract: The genome of the Rose yellow leaf virus (RYLV) has been determined to be 3918 nucleotides containing seven open reading frames (ORFs). ORF1 encodes a 27 kDa peptide (p27). ORF2 shares a common start codon with ORF1 and continues through the amber stop codon of p27 to encode a 87 kDa (p87) protein that has amino acid similarity to the RNA dependent RNA polymerase (RdRp) of members of the family Tombusviridae. ORFs 3 and 4 encode proteins of 8 and 7 kDa respectively that have no significant amino acid similarity to known viral sequences. ORF5 encodes a 6 kDa (p6) protein that has similarity to movement proteins of the Tombusviridae. ORF5A has no conventional start codon and overlaps with p6. A putative +1 frame shift mechanism allows p6 translation to continue through the stop codon and results in a 12 kDa protein that has high homology to the carmovirus p13 movement protein. The 37 kDa protein encoded by ORF6 has amino acid sequence similarity to coat proteins (CP) of Tombusviridae. ORF7 encodes a 7 kDa protein with no significant amino acid similarity to known viral proteins. Phylogenetic analyses of the RdRp amino acid sequences grouped RYLV together with the unclassified Rosa rugosa leaf distortion virus (RrLDV), Pelargonium line pattern virus (PLPV), and Pelargonium chlorotic ring pattern virus (PCRPV) in a distinct subgroup of the family Tombusviridae.