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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Soybean Genomics & Improvement Laboratory » Research » Publications at this Location » Publication #300604

Research Project: Discovery and Introgression of Disease Resistance Genes into Phaseolus vulgaris

Location: Soybean Genomics & Improvement Laboratory

Title: SSR DNA markers linked with Broad-Spectrum rust resistance in common bean discovered by bulk segregant analysis using a large set of SNP markers

Author
item SHIN, SEONG-HYU - Rural Development Administration - Korea
item Song, Qijian
item Cregan, Perry
item Pastor Corrales, Marcial - Talo

Submitted to: Bean Improvement Cooperative Annual Report
Publication Type: Trade Journal
Publication Acceptance Date: 3/15/2014
Publication Date: 5/5/2014
Citation: Shin, S., Song, Q., Cregan, P.B., Pastor Corrales, M.A. 2014. SSR DNA markers linked with Broad-Spectrum rust resistance in common bean discovered by bulk segregant analysis using a large set of SNP markers. Bean Improvement Cooperative Annual Report. 57:187-188.

Interpretive Summary:

Technical Abstract: DNA markers are invaluable plant breeding tools that can be used in the development of new crop cultivars with disease resistance. We wanted to develop the capacity for marker-assisted selection using the broad-spectrum rust resistance trait present in Mesoamerican common bean PI 310762. This common bean was resistant to 89 of the 90 virulent strains of the rust pathogen used in this study. Moreover, PI 310762 was also resistant to all the strains of the rust pathogen that render susceptible all ten known genes that confer resistance to the bean rust pathogen. In this study we used two technologies, called bulk segregant analysis and single nucleotide polymorphism genotyping, to identify eight DNA markers, known as simple sequence repeats that were closely linked with the rust resistance in PI 310762. Some of these markers are located very close and are flanking the resistance trait in PI 310762. Thus, these markers can be used with great efficiency to monitor the presence of the rust resistance trait from PI 310762. These markers, which are easy to visualize in most laboratories of the world using marker assisted selection, will benefit breeders in developing and developed nations wishing to transfer this very important rust resistance trait to their local varieties.