Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 11/7/2013
Publication Date: 12/3/2013
Citation: Dahleen, L.S. 2013. 2013 North Dakota transgenic barley research and FHB nursery report. In: Canty, S., Clark, A., Salat, Y., Van Sanford, D., editors. Proceedings of the National Fusarium Head Blight Forum, East Lansing, MI/Lexington, KY: U.S. Wheat and Scab Initiative. National Fusarium Head Blight Forum, December 3-5, 2013, Milwaukee, WI. p. 65.
Technical Abstract: Research continues to develop and test new transgenic plants using genes provided by collaborators. As lines are developed in Golden Promise, they are crossed to Conlon for field testing. Transgenic lines developed in Conlon are being crossed to resistant lines developed by the breeding programs. Crosses between Conlon and five Golden Promise transgenic plants carrying different transgenes were begun in 2013. At least two of these lines will be ready for field tests in 2014. Crosses between Quest or ND20448 and two Conlon lines carrying either a tlp or a Tri12 transgene have continued, to determine whether the transgenes add to resistance from the breeding programs. Lines from this program should be ready for testing in 2014. The 2013 North Dakota transgenic field trials consisted of 15 barley lines, tested in three misted and three non-misted replicates. Plots were sown on May 29, 2013 in hill plots with 10 seed per hill spaced at 30 cm, and all plots were inoculated using the grain spawn method at heading. Lines included Conlon, Golden Promise, the resistant checks Quest and CI4196, four transgenic-null pairs derived from crosses between primary Golden Promise transgenics and Conlon, and one Golden Promise primary transgenic-null pair. FHB severity was evaluated approximately three weeks after anthesis, by counting the total and infected number of seed on ten randomly selected spikes per row. DON concentrations in the barley samples were determined by gas chromatography with electron capture detection using the method of Tacke and Casper. FHB and DON data were analyzed by SAS (SAS Institute, Cary, NC) with means adjusted for the nearest checks. Average FHB severity was 10% over all six replicates, 7% in the non-irrigated plots and 12% in the irrigated plots. This material is based upon work supported by the US Department of Agriculture. This is a cooperative project with the US Wheat & Barley Scab Initiative.