Skip to main content
ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Soybean Genomics & Improvement Laboratory » Research » Publications at this Location » Publication #299638

Research Project: Developing Soybean and Other Legumes with Resistance to Pathogens and Assessing the Biosafety of Transgenic Soybean

Location: Soybean Genomics & Improvement Laboratory

Title: Examination of the abscission-associated transcriptomes for soybean, tomato, and Arabidopsis highlights the conserved biosynthesis of an extensible extracellular matrix and boundary layer

Author
item Tucker, Mark
item Yang, Ronghui
item SUNDARESAN, SRIVIGNESH - Agricultural Research Organization Of Israel
item MEIR, SHIMON - Agricultural Research Organization Of Israel
item KIM, JOONYUP - University Of Maryland
item PHLOSOPH-HADAS, SONIA - Agricultural Research Organization Of Israel

Submitted to: Frontiers in Plant Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/23/2015
Publication Date: 12/15/2015
Citation: Tucker, M.L., Yang, R., Sundaresan, S., Meir, S., Kim, J., Phlosoph-Hadas, S. 2015. Examination of the abscission-associated transcriptomes for soybean, tomato, and Arabidopsis highlights the conserved biosynthesis of an extensible extracellular matrix and boundary layer. Frontiers in Plant Science. 6(1109):1-15.

Interpretive Summary: We are interested in the identification of small proteins that may be involved in signaling events in soybean. PAR1 expression was noticed because it fits our criteria for a small protein of unknown function that could participate in signaling a change in development or a defense response to pathogens. PAR1 is strongly expressed during abscission, leaf and flower drop, and during infection of soybean roots by soybean cyst nematode. PAR1 may be involved in cell separation events that occur during abscission and nematode infection or in the signaling of a defense response. A better understanding of signals involved in soybean development and nematode infection of soybean roots will greatly improve the ability of scientists and industrial partners to control nematode infection of soybean and other agriculturally important plants.

Technical Abstract: PAR1, photoassimilate-responsive-1, was originally identified in a subtraction library prepared from leaves of transgenic tobacco plants, ppa-1, that accumulate high concentrations of sucrose in source leaves. PAR1 genes are highly conserved in both dicots and monocots. All appear to have a signal peptide for translation on the endoplasmic reticulum and after cleavage encode a relatively small protein of <20 kDa. In dicots there are two clearly separated classes of PAR1 genes. One class of genes is strongly and specifically up-regulated during abscission processes in soybean and tomato. The other class is expressed more broadly in the plant at lower levels. PAR1 expression in abscission is early and follows a pattern of expression similar to cell wall depolymerizing proteins but a different pattern than abscission-associated pathogen-related and senescence-associated genes. The abscission expressed genes are also up-regulated during infection by soybean cyst nematodes, a process that also includes cell wall depolymerization as well as expression of defense genes.