Submitted to: Acta Phytopathologica Sinica
Publication Type: Abstract only
Publication Acceptance Date: 5/1/2013
Publication Date: 8/9/2013
Citation: Qiu, D., Vandemark, G.J., Chen, W. 2013. Comparative transcriptome analysis between the necrotrophic pathogens Sclerotina sclerotiorum and S. trifoliorum. Acta Phytopathologica Sinica. 43 (Suppl.):227. Interpretive Summary: Abstract Only
Technical Abstract: Sclerotinia sclerotiorum and S. trifoliorum cause white mold on many economically important crops. S. trifoliorum mainly infects cool season legumes with a host range of about 40 plant species, whereas the host range of S. sclerotiorum encompasses more than 400 plant species including all the host species of S. trifoliorum. S. sclerotiorum has been extensively studied and its genome sequences are available. However, relatively little is known about S. trifoliorum. We compared the transcriptome of S. trifoliorum with that of S. sclerotiorum in order to gain a better understanding of the biology of both species. A total of 23133 unique transcripts with average length of 439 bases (10.1 Mb genome coverage) were obtained from S. sclerotiorum, whereas 21043 unique transcripts with average length of 418 bases (8.8 Mb genome coverage) were obtained from S. trifoliorum. Approximately 60% of the transcripts were common between the two species, and about 43% of the transcripts were genes with known functions for both species. Among 1411 orthologous contigs (transcripts with more than one read), 147 (10%) were highly (> 3 folds) expressed in S. trifoliorum than in S. sclerotiorum, and 173 (12%) were highly expressed in S. sclerotiorum than in S. trifoliorum. Approximately 140 transcripts from each species were found in the noncoding regions in the annotated genome of Sclerotinia sclerotiorum. Fifteen transcripts in the noncoding region were shared by both species. Additionally, differences in expressed genes involved in pathogenesis like oxalate biosynthesis and endopolygalacturonases were detected between the two species.