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ARS Home » Plains Area » Fargo, North Dakota » Edward T. Schafer Agricultural Research Center » Food Animal Metabolism Research » Research » Publications at this Location » Publication #284793

Title: Generation of antibodies and development of environmental application of immunoassays for PBDEs

item Shelver, Weilin

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 10/17/2012
Publication Date: 3/17/2013
Citation: Shelver, W.L. 2013. Generation of antibodies and development of environmental application of immunoassays for PBDEs [abstract]. The Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy, March 17-21, 2013, Philadelphia, PA.

Interpretive Summary:

Technical Abstract: Polybrominated diphenyl ethers (PBDEs) are utilized as flame retardants and are ubiquitous environmental contaminants. The three major formulations of PBDEs used are pentaBDEs, octaBDEs, and decaBDEs each containing different distributions of the many congeners possible for PBDE. Because of their physical-chemical similarity to other persistent organic chemicals (ie dioxins and PCBs), their accumulation in the environment is of concern and some of the formulations have been banned in many countries. Hapten syntheses and antibody generation toward PBDE will be described. In addition, a comparison of different formats of immunoassays for PBDEs and their application toward environmental sample analyses will be discussed. In all, the immunoassays are rapid, permitting high throughput. The assay gave good recoveries of PBDEs from spiked water samples and a very small within and between day variance. The cleanup methods prior to immunoassay were matrix dependent; no/minimal pretreatment was needed for environmental water samples; while other types of samples required various degrees of cleanup. Validation with instrumentation method demonstrated good correlation between immunoassay and GC-MS measurements. Analysis of a wide variety of environmental samples by both immunoassay and GC-MS demonstrated immunoassays provide a timely and cost effective method to screen for PBDEs.