|THOMMA, BART - Wageningen University And Research Center
Submitted to: Complete Book
Publication Type: Book / Chapter
Publication Acceptance Date: 11/20/2011
Publication Date: 1/5/2012
Citation: Bolton, M.D., Thomma, B.P. 2012. Plant Fungal Pathogens: Methods and Protocols. New York, NY: Humana Press. p 648.
Technical Abstract: Over the course of evolution, fungi have adapted to occupy specific niches, from symbiotically inhabiting the flora of the intestinal tract of mammals to saprophytic growth on leaf litter resting on the forest floor. In plant Fungal Pathogens: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study fungal plant pathogens. These include methods and techniques for model systems such as Arabidopsis thaliana as well as crop plants, aspect of fungal biology, genome annotation, next-generation sequencing and fungal transformation and molecular tools for disease and/or pathogen quantification that are critical for revealing the role for a fungal gene of interest in disease development. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily re-producible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Fungal Pathogens: Methods and Protocols seeks to aid scientists in the further study in current techniques that cover a wide-range of methods to study molecular aspects of pathogenesis.