|Xu, Liangsheng - Washington State University|
Submitted to: Pisum Genetics
Publication Type: Abstract Only
Publication Acceptance Date: 4/5/2012
Publication Date: N/A
Technical Abstract: Sclerotinia sclerotiorum causes white mold disease on pea and on many other economically important pulse, vegetable and field crops, demonstrating a non-host-specific pathogenic mechanism. Despite extensive studies on this pathogen, its pathogenic mechanisms are still incompletely understood. In order to gain insight in understanding its non-specific host-pathogen interactions, Agrobacterium-mediated transformation (AMT) was used to generate random mutations and to identify potential virulence/pathogenicity factors in S. sclerotiorum. Among several hundreds of AMT transformants screened, two stable mutants showed significantly less virulence in comparison with the wild type strain as measured by colonizing pea leaves in detached leaf assays. Hybridization and inverse PCR analyses showed that the mutation was due to a single T-DNA insertion at the gene Cu-Zn-superoxide dismutase (SOD, SS1G00699) of S. sclerotiorum. Phenotypic and genotypic characterization of the SOD mutants will be presented.