Submitted to: Analytical Chemistry
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/3/2011
Publication Date: 10/3/2011
Citation: Meimaridou, A., Kalachova, K., Shelver, W.L., Franek, M., Pulkrabova, J., Haasnoot, W., Nielen, M.W.F. 2011. Multiplex screening of persistent organic pollutants in fish using spectrally encoded microspheres. Analytical Chemistry. 83:8696-8702. Interpretive Summary: The presence of various contaminants in food is a matter of increasing concern to the public. Consequently, it is necessary to develop analytical methods capable of determining if these contaminates are present or not. Furthermore, this must be done in a cost efficient manner. In this study a method was developed to determine three types of contaminants in fish of various types in one simple assay using a simple sample preparation. This assay showed the ability to detect these contaminates with sensitivity to meet regulatory limits. The method is facilitated by the use of a technique known as flow cytometric immunoassay (FCIA) which allows the high sensitivity and specificity needed for the three types of food contaminants in the complex matrix of fish meat.
Technical Abstract: Persistent organic pollutants (POPs) are food contaminants of global public health concern and known to be carcinogenic and endocrine disruptors. Their monitoring is essential and an easy-to-use, rapid and affordable multi-analyte screening method with simplified sample preparation can be a valuable tool prior to instrumental analysis. For this purpose, a flow cytometric immunoassay (FCIA), based on a spectrally-encoded microbead technology, was developed for the multiplex detection of polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (BDEs) in buffer and fish extracts. The sensitivities of the assays in the 3-plex FCIA format were similar to the individual FCIAs for the marker compounds benzo[a]pyrene, 1,1'-biphenyl, 3,3',4,4'-tetrachloro- and benzene, 2,4-dibromo-(2,4-dibromophenoxy) in buffer with IC50 values of 0.4, 20 and 2 ug L-1, respectively. Apart from the three markers, we could detect at least 14 other POPs. Extracts of fish with different fat content, prepared with a simplified extraction and clean-up procedure had an insignificant influence on the overall 3-plex FCIA performance, with the exception of some impact on the PAHs detection. The performance of the 3-plex FCIA, in combination with the simple extraction procedure, is adequate for regulatory control in accordance with the required limits.