Submitted to: European Journal of Plant Pathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/10/2011
Publication Date: 2/17/2012
Citation: Ali, H., Haq, A., Shah, T., Chen, W. 2012. Validation of molecular markers for resistance among Pakistani chickpea germplasm to races of Fusarium oxysporum f. sp. ciceris. European Journal of Plant Pathology. 132:237-244. DOI 10.1007/s10658-011-9868-1. Interpretive Summary: Fusarium wilt is an important disease of chickpea in Pakistan and breeding for resistant cultivars is critical for managing the disease. Molecular markers have been developed for resistance to races of the pathogen Fusarium oxysporum f.sp. ciceris. However, the applicability of these markers in Pakistani chickpea germplasm is unknown. Research was carried out to validate those molecular markers for wilt resistance in Pakistani chickpea lines. Most of the microsatellite markers showed good correlation with disease resistance against different races and may be used effectively in resistance breeding except those markers for race 3. The reason for the exception of race 3 might be that race 3 is actually Fua different Fusarium species as reported recently, and resistance to race 3 might involve some other major resistance genes. In addition, three Pakistani mutant lines (97477, CM444/92 and CM368/93) were found to have complete resistance against races 1A, 2 and 4 and high levels of resistance (11-19% disease incidence) for F. proliferatum (race 3). These lines will be useful as parents for developing new chickpea germplasm with resistance to Fusarium wilt.
Technical Abstract: DNA markers in chickpea have been identified against different races of Fusarium oxysporum f.sp. ciceris (Foc), but validation of these markers is essential for their effective use in resistant breeding. In view of this, different simple sequence repeats (SSR) markers were analysed in Pakistani germplasm including induced mutants and some local lines. Most of the SSR markers showed good correlation with phenotypic evaluation of genotypes with different races and may be used effectively in resistance breeding except those markers for race 3. Markers for race 3 showed deviation from phenotypic data. The reason might be that race 3 is actually Fusarium proliferatum as reported recently and resistance to race 3 might involve some other major resistance genes. Poor correlation of markers with foc-3 on LG2 in our study and a recent report of independent segregation of foc2 and foc3 in near isogenic lines suggested that linkage distances among different resistant genes need further investigation. Moreover three Pakistani mutant lines (97477, CM444/92 and CM368/93) were found to have complete resistance against races 1A, 2 and 4 and high levels of resistance (11-19% disease incidence) for F. proliferatum (race 3). These genotypes are valuable sources of resistance against Fusarium for resistance breeding programmes.