|Moorman, Thomas - Tom|
|SOUPIR, MICHELLE - Iowa State University|
|GARDER, JASON - Iowa State University|
Submitted to: ASA-CSSA-SSSA Annual Meeting Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 10/16/2011
Publication Date: 10/16/2011
Citation: Moorman, T.B., Soupir, M., Garder, J. 2011. Transport of tylosin and tylosin-resistance genes in subsurface drainage water from manured fields. American Society of Agronomy Annual Meetings [abstracts]. ASA-CSSA-SSSA Annual Meeting, Oct. 16-19, 2011, San Antonio, TX. CD-ROM.
Technical Abstract: Animal agriculture appears to contribute to the spread of antibiotic resistance genes, but few studies have quantified gene transport in agricultural fields. The transport of tylosin, tylosin-resistance genes (erm B, F, A) and tylosin-resistant Enterococcus were measured in tile drainage water from plots treated with manure from tylosin-treated swine. Enterococcus measured after fall manure application reached 700 cells/g soil, but tylosin-resistant Enterococcus accounted for less than 10% of the total soil population. Enterococcus soil populations declined over the winter. Tylosin-resistant bacteria were detected in tile drainage water only in the early spring and tylosin was sporadically detected in drainage water at concentrations below 1 ng/L. The abundance of erm genes was measured by qPCR and those results will be discussed.