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ARS Home » Southeast Area » Little Rock, Arkansas » Microbiome and Metabolism Research Unit » Research » Publications at this Location » Publication #270301

Title: Velutin reduces lipopolysaccharide-induced proinflammatory cytokine TNFa and IL-6 production by inhibiting NF-Kappa B activation

Author
item XIE, CHENGHUI - Arkansas Children'S Nutrition Research Center (ACNC)
item KANG, JIE - Arkansas Children'S Nutrition Research Center (ACNC)
item LI, ZHIMIN - Shanghai Institute Of Pharmaceutical Industry
item NAGARAJAN, SHANMUGAM - Arkansas Children'S Nutrition Research Center (ACNC)
item SCHAUSS, ALEXANDER - Aimbr Life Sciences
item WU, TONG - Shanghai Institute Of Pharmaceutical Industry
item WU, XIANLI - Arkansas Children'S Nutrition Research Center (ACNC)

Submitted to: Federation of American Societies for Experimental Biology Conference
Publication Type: Abstract Only
Publication Acceptance Date: 12/15/2010
Publication Date: 4/1/2011
Citation: Xie, C., Kang, J., Li, Z., Nagarajan, S., Schauss, A.G., Wu, T., Wu, X. 2011. Velutin reduces lipopolysaccharide-induced proinflammatory cytokine TNFa and IL-6 production by inhibiting NF-Kappa B activation. The FASEB Journal. 25(Meeting Abstract):772.13.

Interpretive Summary: Recent studies have shown that some flavonoids are modulators of proinflammatory cytokine expression. Velutin, an uncommon flavone isolated from acai (Euterpe oleraceas) berry, was tested for the effects in reducing LPS-induced inflammatory cytokine production. Velutin exhibited the strongest effects in reducing both TNF-alpha and IL-6 dose-dependently in the two type cells. Velutin also strongly inhibited NF-Kappa B activation. In line with these findings, velutin blocked the degradation of I Kappa B, a key step in NF-Kappa B activation. In conclusion, velutin effectively inhibited proinflammatory cytokine production in low micromole levels by inhibiting NF-Kappa B activation through blocking the degradation of I Kappa B.

Technical Abstract: Recent studies have shown that some flavonoids are modulators of proinflammatory cytokine expression. Velutin, an uncommon flavone isolated from acai (Euterpe oleraceas) berry, was tested for the effects in reducing LPS-induced TNFa and IL-6 production in RAW 264.7 peripheral macrophages and peritoneal macrophages from C57/BL mice. Three other flavones, luteolin, apigenin and chrysoeriol, were included for comparative purposes. Velutin exhibited the strongest effects in reducing both TNF-alpha and IL-6 dose-dependently in the two type cells, followed by apigenin, luteolin and chrysoeriol. The number and/or position of hydroxyl/methoxy groups appear to be important factors in determining the anti-inflammatory effects of the flavones. Velutin also strongly inhibited NF-Kappa B activation measured by secreted alkaline phosphatase (SEAP) reporter assay. In line with these findings, velutin blocked the degradation of I'B, a key step in NF-Kappa B activation. In conclusion, velutin effectively inhibited proinflammatory cytokine production in low micromole levels by inhibiting NF-Kappa B activation through blocking the degradation of I Kappa B.