Location: Southern Insect Management ResearchTitle: cDNA microarray analysis of gene expression in insecticide-susceptible and –resistant Tarnished Plant bug Author
|Zhu, Yu Cheng|
Submitted to: National Cotton Council Beltwide Cotton Conference
Publication Type: Proceedings
Publication Acceptance Date: 3/1/2011
Publication Date: 3/1/2011
Citation: Guo, Z., Zhu, Y., Luttrell, R.G. 2011. cDNA microarray analysis of gene expression in insecticide-susceptible and –resistant Tarnished Plant bug. National Cotton Council Beltwide Cotton Conference. January 4-7, 2011, Atlanta, Gerogia, 2011. p.876. 2011CDROM. Interpretive Summary: Tarnished plant bug, Lygus lineolaris (Palisot de Beauvois) is an important cotton pest in the Mid-south region of the United States. Management of the tarnished plant bug relies almost exclusively on chemical control strategies. Over the years, surviving populations of the tarnished plant bug have increasingly become resistant to these chemical insecticides and farmers have had to increase the dose to maintain chemical efficacy against target pests. Plant bug populations with high levels of resistance to the organophosphate insecticide (acephate) were found in the Hill and Delta regions of Arkansas, Louisiana and Mississippi in 2005 and 2006. In an attempt to understand insecticide resistance mechanism in the tarnished plant bug, microarray was used to identify a differential gene expression pattern associated with different susceptibilities to acephate between a susceptible laboratory colony and the field-collected resistant strains. Several genes were up or down-regulated in the field-collect acephate-resistant strains. One up-regulated esterase gene was validated by using semi-qutatitative RT-PCR. Other up- or down-regulated genes will also be validated and characterized by real-time PCR in our future work.
Technical Abstract: Insecticide resistance in the tarnished plant bug is most likely associated with increased detoxification gene expression. To better understand the molecular mechanisms of insecticide resistance, an insecticide-susceptible laboratory colony and several resistant field-collected strains of Lygus lineolaris were subjected to a microarray analysis. We found 14 up-regulated genes and 12 down-regulated genes in acephate-resistant strain. One easterase up-regulated gene has been confirmed by semi-quantitative RT-PCR. This cDNA microarray gene expression information could be used to identify new genes that are associated with insecticide resistance development in the tarnished plant bug.