Submitted to: Plant and Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: 10/25/2010
Publication Date: 1/15/2011
Citation: Pan, Y.-B. 2011. Inheritance of SSR DNA markers in a self progeny population of the Louisiana sugarcane cultivar LCP 85-384 [abstract]. Plant and Animal Genomes XIX Conference, January 15-19, 2011. San Diego, CA. Available: http://www.intl-pag.org/19/abstracts/P05d_PAGXIX_358.html
Technical Abstract: To develop a genetic linkage map for Louisiana sugarcane germplasm, two tassels of LCP 85-384, a BC4 progeny of US56-15-6, were self-pollinated in spatially isolated area after all pre-dehisced florets were trimmed. Two hundred and eighty-eight self progeny were randomly picked up from the germinating seedlings to make up the mapping population. The genetic purity of the mapping population was verified by genotyping with 19 SSR markers on the ABI3730XL platform. Sixty-one SSR DNA fragments were amplified from LCP 85-384. These 61 DNA fragments were found to segregate among 279 self progeny, 22 fragments segregated as single dose markers (3:1), 10 as double dose (15:1), 11 as triple dose (63:1), 7 as quadric dose (255:1), and 11 as being non-interpretable. None of the 279 self progeny amplified any non-LCP 85-384 SSR DNA fragment. However, 31 non-LCP 85-384 SSR DNA fragments were amplified from the other nine progeny by no fewer than six and as many as 13 SSR markers. The larger stature of these nine progeny was more like that of the progeny of commercial crosses than the self progeny of LCP 85-384. The genetic purity of this mapping population was determined to be 97%. The nine progeny (3%) that produced non-LCP 85-384 SSR DNA fragments were considered to be off-types and were excluded from the mapping population to ensure scientific integrity and avoid potential flaws in the construction of linkage maps and identification of QTL markers.