Submitted to: National Cotton Council Beltwide Cotton Conference
Publication Type: Abstract Only
Publication Acceptance Date: 1/6/2011
Publication Date: 1/6/2011
Citation: Fang, D.D., Stetina, S.R. 2011. Are Renari from Gossypium aridum and Renlon from G. longicalyx Duplicate Genes?. National Cotton Council Beltwide Cotton Conference. CD ROM. Interpretive Summary:
Technical Abstract: Reniform nematode (Rotylenchulus reniformis) has become a major pest of cotton in the mid-south area of the United States. Resistance genes, Renari and Renlon from Gossypium aridum and G. longicalyx, respectively, have been identified and introgressed into Upland cotton. Using plant materials containing either Renari or Renlon gene as parents, we developed an F2 population of 184 progeny for genetic evaluation of nematode resistance. The response of F2 plants to nematode inoculation was evaluated in a growth chamber. Microsatellite markers BNL2662 and BNL3279 were analyzed to assist selection of proper parents and F1 plants, and to study the segregation of the resistance genes among the F2 progeny. Our results suggested that Renari and Renlon were duplicate genes with Renari residing on chromosome 21, and Renlon on chromosome 11. F2 plants containing either Renari or Renlon had significantly fewer nematodes than the susceptible Upland cotton genotype. No significant difference in nematode resistance was found between plants containing Renari and those having Renlon, indicating that these two genes may have similar resistance mechanisms. Pyramiding Renari and Renlon further enhanced plant resistance to reniform nematodes.