Skip to main content
ARS Home » Plains Area » Fargo, North Dakota » Edward T. Schafer Agricultural Research Center » Food Animal Metabolism Research » Research » Publications at this Location » Publication #260430

Title: Effects of liquid swine manure on sorption of 17ß-estradiol to soil

Author
item ZITNICK, KIM - North Dakota State University
item Hakk, Heldur
item Shappell, Nancy
item DESUTTER, THOMAS - North Dakota State University
item CASEY, FRANCIS - North Dakota State University
item KHAN, EAKALAK - North Dakota State University

Submitted to: ASA-CSSA-SSSA Annual Meeting Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 6/20/2009
Publication Date: 11/3/2009
Citation: Zitnick, K., Hakk, H., Shappell, N.W., Desutter, T., Casey, F.S., Khan, E. 2009. Effects of liquid swine manure on sorption of 17ß-estradiol to soil. ASA-CSSA-SSSA Annual Meeting, November 1-5, 2009, Pittsburgh, PA. Abstract #52904. Available http://a-c-s.confex.com/crops/ 2009am/webprogram/Paper52904.html.

Interpretive Summary:

Technical Abstract: Estrogens are potent endocrine disrupting chemicals and can be found in the environment. Animal manure can contain estrogenic hormones, specifically 17'-estradiol (E2). A common manure management practice is to contain liquid manure in a manure storage ponds or lagoons, which is then applied on or injected into the land surface. Laboratory studies have demonstrated that E2 binds readily and strongly to soil and biodegrades within hours. However, several field studies have frequently detected E2 in the environment, which suggest moderate mobility and persistence. Therefore, it was hypothesized that E2 binds to colloidal organic carbon (COC) and dissolved organic carbon (DOC) within the lagoon manure, which may facilitate its mobility and perhaps its persistence. The objective of this study was to determine if COC and DOC from lagoon manure affect the sorption and potential mobility of E2 in soil. Batch experiments were used to determine the sorption distribution of E2 in soil with lagoon waste solution and compared to batch experiments conducted with only a 0.1M CaCl2 solution. A series of batch experiments were made up of four concentrations of 14C labeled E2 (ranging from 0.01-0.7 µCi per vial) and two sterile soils of identical texture but different organic carbon contents (1.35 and 0.32%). Batches vials were rotated, sampled at nine set time points (0.17, 0.33, 1, 2, 3, 7, 14, 21, 28 days) for liquid scintillation counting (LSC) and thin layer chromatography analysis. To determine what manure organic carbon fraction (i.e., DOC or COC) that E2 preferentially associated with, a lagoon sample spiked with 14C E2 was run through a 1.5µm filter and a 1kD ultrafilter. The filtrate was sent to LSC for quantifying 14C E2 amounts.