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ARS Home » Pacific West Area » Davis, California » Western Human Nutrition Research Center » Obesity and Metabolism Research » Research » Publications at this Location » Publication #259097

Title: The human serum metabolome

Author
item PSYCHOGIOS, NIKOLAOS - University Of Alberta
item HAU, DAVID - University Of Alberta
item PENG, JUN - University Of Alberta
item GUO, AN CHI - University Of Alberta
item MANDAL, RUPARSI - University Of Alberta
item BOUATRA, SOUHAILA - University Of Alberta
item SINELNIKOV, IGOR - University Of Alberta
item KRISHNAMURTHY, RAMANARAYAN - University Of Alberta
item EISNER, ROMAN - University Of Alberta
item GAUTAM, BIJAYA - University Of Alberta
item YOUNG, NELSON - University Of Alberta
item XIA, JINAGUO - University Of Alberta
item KNOX, CRAIG - University Of Alberta
item DONG, EDISON - University Of Alberta
item HUANG, PAUL - University Of Alberta
item HOLLANDER, ZSUZSANNA - University Of British Columbia
item Pedersen, Theresa
item SMITH, STEVEN - Pennington Biomedical Research Center
item BAMFORTH, FIONA - University Of Alberta
item GREINER, RUSS - University Of Alberta
item MCMANUS, BRUCE - University Of British Columbia
item Newman, John
item GOODFRIEND, THEODORE - University Of Wisconsin
item WISHART, DAVID - University Of Alberta

Submitted to: PLoS One
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/18/2011
Publication Date: 2/16/2011
Publication URL: http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0016957
Citation: Psychogios, N., Hau, D.D., Peng, J., Guo, A., Mandal, R., Bouatra, S., Sinelnikov, I., Krishnamurthy, R., Eisner, R., Gautam, B., Young, N., Xia, J., Knox, C., Dong, E., Huang, P., Hollander, Z., Pedersen, T.L., Smith, S.R., Bamforth, F., Greiner, R., Mcmanus, B., Newman, J.W., Goodfriend, T., Wishart, D.S. 2011. THE HUMAN SERUM METABOLOME. PLoS One. 6(2): e16957.

Interpretive Summary: Continuing improvements in analytical technology along with an increased interest in performing comprehensive, quantitative metabolic profiling, is leading to increased interest pressures within the metabolomics community to develop centralized metabolite reference resources for certain clinically important biofluids, such as cerebrospinal fluid, urine and blood. As part of an ongoing effort to systematically characterize the human metabolome through the Human Metabolome Project, we have undertaken the task of characterizing the human serum metabolome. In doing so, we have combined targeted and non-targeted NMR, GC-MS and LC-MS methods with computer-aided literature mining to identify and quantify a comprehensive, if not absolutely complete, set of metabolites commonly detected and quantified (with today‘s technology) in the human serum metabolome. Our use of multiple metabolomics platforms and technologies allowed us to substantially enhance the level of metabolome coverage while critically assessing the relative strengths and weaknesses of these platforms or technologies. Tables containing the complete set of 4229 human serum compounds, their concentrations, related literature references and links to their known disease associations are freely available at http://www.serummetabolome.ca.

Technical Abstract: Continuing improvements in analytical technology along with an increased interest in performing comprehensive, quantitative metabolic profiling, is leading to increased interest pressures within the metabolomics community to develop centralized metabolite reference resources for certain clinically important biofluids, such as cerebrospinal fluid, urine and blood. As part of an ongoing effort to systematically characterize the human metabolome through the Human Metabolome Project, we have undertaken the task of characterizing the human serum metabolome. In doing so, we have combined targeted and non-targeted NMR, GC-MS and LC-MS methods with computer-aided literature mining to identify and quantify a comprehensive, if not absolutely complete, set of metabolites commonly detected and quantified (with today‘s technology) in the human serum metabolome. Our use of multiple metabolomics platforms and technologies allowed us to substantially enhance the level of metabolome coverage while critically assessing the relative strengths and weaknesses of these platforms or technologies. Tables containing the complete set of 4229 human serum compounds, their concentrations, related literature references and links to their known disease associations are freely available at http://www.serummetabolome.ca.