The human serum metabolome

Authors: PSYCHOGIOS, NIKOLAOS - University Of Alberta; HAU, DAVID - University Of Alberta; PENG, JUN - University Of Alberta; GUO, AN CHI - University Of Alberta; MANDAL, RUPARSI - University Of Alberta; BOUATRA, SOUHAILA - University Of Alberta; SINELNIKOV, IGOR - University Of Alberta; KRISHNAMURTHY, RAMANARAYAN - University Of Alberta; EISNER, ROMAN - University Of Alberta; GAUTAM, BIJAYA - University Of Alberta; YOUNG, NELSON - University Of Alberta; XIA, JINAGUO - University Of Alberta; KNOX, CRAIG - University Of Alberta; DONG, EDISON - University Of Alberta; HUANG, PAUL - University Of Alberta; HOLLANDER, ZSUZSANNA - University Of British Columbia; Pedersen, Theresa; SMITH, STEVEN - Pennington Biomedical Research Center; BAMFORTH, FIONA - University Of Alberta; GREINER, RUSS - University Of Alberta; MCMANUS, BRUCE - University Of British Columbia; Newman, John; GOODFRIEND, THEODORE - University Of Wisconsin; WISHART, DAVID - University Of Alberta

Submitted to: PLOS ONE
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/18/2011
Publication Date: 2/16/2011
Citation: Psychogios, N., Hau, D.D., Peng, J., Guo, A., Mandal, R., Bouatra, S., Sinelnikov, I., Krishnamurthy, R., Eisner, R., Gautam, B., Young, N., Xia, J., Knox, C., Dong, E., Huang, P., Hollander, Z., Pedersen, T.L., Smith, S.R., Bamforth, F., Greiner, R., Mcmanus, B., Newman, J.W., Goodfriend, T., Wishart, D.S. 2011. THE HUMAN SERUM METABOLOME. PLoS One. 6(2): e16957.

Interpretive Summary: Continuing improvements in analytical technology along with an increased interest in performing comprehensive, quantitative metabolic profiling, is leading to increased interest pressures within the metabolomics community to develop centralized metabolite reference resources for certain clinically important biofluids, such as cerebrospinal fluid, urine and blood. As part of an ongoing effort to systematically characterize the human metabolome through the Human Metabolome Project, we have undertaken the task of characterizing the human serum metabolome. In doing so, we have combined targeted and non-targeted NMR, GC-MS and LC-MS methods with computer-aided literature mining to identify and quantify a comprehensive, if not absolutely complete, set of metabolites commonly detected and quantified (with today‘s technology) in the human serum metabolome. Our use of multiple metabolomics platforms and technologies allowed us to substantially enhance the level of metabolome coverage while critically assessing the relative strengths and weaknesses of these platforms or technologies. Tables containing the complete set of 4229 human serum compounds, their concentrations, related literature references and links to their known disease associations are freely available at http://www.serummetabolome.ca.

Technical Abstract: Continuing improvements in analytical technology along with an increased interest in performing comprehensive, quantitative metabolic profiling, is leading to increased interest pressures within the metabolomics community to develop centralized metabolite reference resources for certain clinically important biofluids, such as cerebrospinal fluid, urine and blood. As part of an ongoing effort to systematically characterize the human metabolome through the Human Metabolome Project, we have undertaken the task of characterizing the human serum metabolome. In doing so, we have combined targeted and non-targeted NMR, GC-MS and LC-MS methods with computer-aided literature mining to identify and quantify a comprehensive, if not absolutely complete, set of metabolites commonly detected and quantified (with today‘s technology) in the human serum metabolome. Our use of multiple metabolomics platforms and technologies allowed us to substantially enhance the level of metabolome coverage while critically assessing the relative strengths and weaknesses of these platforms or technologies. Tables containing the complete set of 4229 human serum compounds, their concentrations, related literature references and links to their known disease associations are freely available at http://www.serummetabolome.ca.