|Dowd, Michael - Mike|
Submitted to: Anticancer Research
Publication Type: Peer reviewed journal
Publication Acceptance Date: 2/13/2009
Publication Date: 6/1/2009
Publication URL: hdl.handle.net/10113/42619
Citation: Huang, Y., Wang, L., Dowd, M.K., Wan, P.J., Lin, Y.C. 2009. (-)-Gossypol reduces invasiveness in metastatic prostate cancer cells. Anticancer Research. 29:2179-2188. Interpretive Summary: Experiments were conducted with (-)-gossypol to demonstrate that the compound slowly the progression of prostate cancer cells. (-)-Gossypol was found to induce a dose-dependent inhibition of invasive activity and cell viability and reduced the level of proteins associated with the control of apoptosis in treated cancer cell lines. These finding illustrate that (-)-gossypol reduces invasion of both the parental MAT-LyLu cells and the isolated MLL cells, suggesting that (-)-gossypol might serve as a chemotherapeutic and/or chemopreventive agent. The results will be of interest to researchers developing cancer therapies.
Technical Abstract: Acquisition of metastatic ability by prostatic cancer cells is the most lethal aspect of prostatic cancer progression. (-)-Gossypol, a polyphenolic compound present in cottonseeds, possesses anti-proliferation and pro-apoptotic effects in various cancer cells. In this study, the differences between MAT-LyLu, rat prostate cancer cells, with a novel isolated sub-line from metastasized tumors in the lungs of MAT-LyLu-bearing Copenhagen rats (MLL cells) were compared with respect to cell growth and invasion. The effects of (-)-gossypol on cell viability, colony formation, invasive ability and cell migration in MAT-LyLu and MLL cells were also evaluated. Results showed that MLL cells displayed higher growth ability, colony formation and aggressive penetration than those of MAT-LyLu cells. MLL cells possess lower protein expression of Bcl-xL and nm23-H1 than those of MAT-LyLu cells, implying differences in invasive ability. Moreover, (-)-gossypol treatment induced a dose-dependent inhibition of invasive activity and cell viability and reduced Bcl-2 and Bcl-xL proteins but induced nm23-H1 protein in both cell lines. These finding illustrated that (-)-gossypol reduced in vitro invasion of both the parental MAT-LyLu cells and the isolated MLL cells, suggesting that (-)-gossypol might serve as a chemotherapeutic and/or chemopreventive agent.