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ARS Home » Plains Area » Fargo, North Dakota » Edward T. Schafer Agricultural Research Center » Animal Metabolism-Agricultural Chemicals Research » Research » Publications at this Location » Publication #235631

Title: Fate of glucuronide conjugated estradiol in soil

item Smith, David
item Hakk, Heldur
item Larsen, Gerald

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 6/3/2008
Publication Date: 10/5/2008
Citation: Shrestha , S. L., Casey, F. X. M., Smith, D. J., Hakk, H.,Larsen, G., and Padmanabhan, G.,2008, Fate of Glucuronide Conjugated Estradiol in Soil.Annual meeting of the Geol. Soc. Am. Agron. Soc. Am., Crop Sci. Soc. Am., and Soil Sci. Soc. Am., Houston, October 5-9, 2008.

Interpretive Summary:

Technical Abstract: The reproductive hormone, 17ß-estradiol (E2), is made more water soluble (polar) in the body by attachment of glucuronide acid to E2, facilitating urinary elimination. The fate of this potentially more mobile polar form of E2 is not well understood. Soil sorption studies were conducted using [14C] 17-ß-estradiol-3-glucuronide (E2G) and a Hamar soil (Sandy, mixed, frigid typic Endoaquolls) for initial concentrations ranging from 0.364 to 28 mg/L. For initial concentrations of 2.8 to 28 mg/L, the aqueous concentrations of E2G rapidly decreased to 11-20% in the first 48 h, with first-order sorption rate constants ranging from 0.55 to 0.67. For the lowest initial concentration of 0.364 mg/L, a higher first-order rate constant of 0.90 was observed. Analysis of the aqueous phase with HPLC indicated that E2G degraded into E2 and other estrogen metabolites. For the first 8 h of the experiment, E2G composed 86-94% of the aqueous concentrations. Also, present in this first 8 h was an unknown polar compound. At 24 h, about 27% of the aqueous fraction was E2, 71% was estrone, and 2% was E2G. During the remainder of the study (up to 672 h) estrone concentrations were at least two times greater than E2 and little or no E2G or polar metabolite were present. Thus, the first 8 h after urinary elimination may be critical for E2G being transported to the receiving water systems. Acetone extraction of removable radiochemical mass from the soil yielded recoveries that ranged from 35% at 4 hr to 93% at 28 days. The acetone-extracted component was determined to be largely estrone, and no E2G was detected. Combustion analyses of residual soil extracts, which represented covalently bound radiochemical to the soil, resulted in recoveries ranging from 7 to 22% for 4 h to 28 d, respectively.