|Holbrook, Carl - Corley|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 12/5/2008
Publication Date: 12/20/2008
Citation: Hong, Y.B., Liang, X.Q., Chen, X.P., Liu, H.Y., Zhou, G.Y., Li, S.X., Wen, S.J., Holbrook Jr, C.C., Guo, B. 2008. A ssr-based genetic linkage map of cultivated peanut (arachis hypogaea l.). Third International Conference on Advances in Arachis through Genomics and Biotechnology, November 4-8, 2008, Hyderabad, India. p. 77.
Interpretive Summary: not required
Technical Abstract: The objective of this study was to construct a molecular linkage map of cultivated tetraploid peanut using simple sequence repeat (SSR) markers derived primarily from peanut genomic sequences, expressed sequence tags (ESTs), and by "data mining" sequences released in GenBank. Three recombinant inbred line (RIL) populations were constructed from three crosses with one common female parental line Yueyou 13, a high yielding Spanish market type. The four parents were screened with 1044 primer pairs designed to amplify SSRs and 901 primer pairs produced clear PCR products. Of the 901 primer pairs, 146, 124 and 64 markers were polymorphic in these populations, respectively, and used in genotyping these RIL populations. Three individual linkage maps were constructed from each population and a composite map based on 93 common loci were created using JoinMap. The composite linkage maps consist of 22 composite linkage groups with 175 SSR markers (including 46 SSRs on the published AA genome maps), representing the 20 chromosomes of A. hypogaea. The total composite map length is 885.4 cM, with an average marker density of 5.8 cM. Segregation distortion was 23.0%, 13.5% and 7.8% of the markers in each population, respectively. These distorted loci tended to cluster on linkage groups 1, 3, 4 and 5. There were only 15 EST-SSR markers mapped due to low polymorphism.