Submitted to: Journal of Federation of American Societies for Experimental Biology
Publication Type: Abstract only
Publication Acceptance Date: 1/7/2009
Publication Date: 4/27/2009
Publication URL: www.fasebj.org
Citation: Shen, C., Yeh, J.K., Dunn, D.M., Cao, J.J., Wang, J. 2009. Synergistic Effect of Green Tea Polyphenols and Vitamin D on Chronic Inflammation-Induced Bone Loss in Female Rats. Journal of Federation of American Societies for Experimental Biology. 23:220.2. Interpretive Summary:
Technical Abstract: Our recent study demonstrated a bone-protective role of green tea polyphenols (GTPs), extracted from green tea, in chronic inflammation-induced bone loss of female rats through reduction of inflammation and oxidative stress. This study further examines effects of GTPs in conjunction with vitamin D (vitD) on chronic-inflammation-induced bone loss of female rats. A 2 (no GTPs vs. GTPs in drinking water) × 2 (no vitD vs. vitD) factorial design using 40 female rats (3-month-old) assigned to 4 groups (n=10/group), LPS implantation (L), L+0.5% GTP (LG), L+vitD (LD), and LPS+0.5% GTP+vitD (LGD) for 12 wks. The results of two-way ANOVA show that 12 weeks supplementation of GTPs in the drinking water increased concentrations of epigallocatechin in urine of rats. GTPs supplementation resulted in a decrease in inflammation index (total white blood cell count, WBC), DNA oxidative damage biomarker (8-hydroxydeoxyguanosine level, 8-OHdG), and bone resorption biomarker (tartrate resistant acid phosphatase, TRAP), no change in bone formation biomarker (osteocalcin, OC), and an increase in bone mineral density (BMD). Similar to GTPs supplementation, vitamin D treatment also led to a reduction of 8-OHdG and TRAP, no change in OC, and an increase in BMD. A combination of GTPs and vitamin D treatments resulted in a synergistic effect on BMD. These results demonstrated that GTPs in conjunction with vitamin D treatment mitigates LPS-induced bone mineral density loss in female rats. Such a bone-synergistic role of GTPs and vitamin D may be, in part, attributed to decreased inflammation and oxidative stress. This study suggests a potentially significant prophylactic role of green tea and vitamin D in bone health of women with chronic inflammation-induced bone loss.