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ARS Home » Midwest Area » Columbia, Missouri » Plant Genetics Research » Research » Publications at this Location » Publication #227083

Title: Responses of the Lichen Photobiont Trebouxia erici to Desiccation and Rehydration (II) Proteomics

Author
item GASULLA, FRANCISCO
item JAIN, RENUKA
item THELAN, JAY
item BARRANO, EVA
item Oliver, Melvin

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 5/16/2008
Publication Date: 7/13/2008
Citation: Gasulla, F., Jain, R., Thelan, J., Barrano, E., Oliver, M.J. 2008. Responses of the Lichen Photobiont Trebouxia erici to Desiccation and Rehydration (II) Proteomics. International Association for Lichenology/American Bryological and Lichenological Society Joint Meeting, July 13-19, 2008, Monterey Peninsula, California. 2008 CDROM.

Interpretive Summary:

Technical Abstract: Lichen desiccation tolerance is associated with cellular protection mechanisms directed against the oxidative stress produced during dehydration and/or rehydration, however, these mechanisms are not well understood. In other poikilohydric organisms, changes in the synthesis of proteins have been implicated in recovery/repair mechanisms following rehydration. In this study we used an isolated strain of the desiccation-tolerant lichen phycobiont Trebouxia erici to analyze protein synthesis during dehydration and rehydration. We employed the strategy of 2-D Difference Gel Electrophoresis (DiGE) coupled with individual protein identification via trypsin digestion (from silver stained replicates) and LC-MS/MS. Two families of proteins increased in response to desiccation: heat shock proteins, HSP90s, that act as chaperones in the non-covalent folding/unfolding of proteins and the assembly/ disassembly of macromolecular structures; and beta-tubulins, cytoskeleton proteins whose synthesis could be important in the recovery of the normal cellular organization upon rehydration. Surprisingly, we detected no change in antioxidant enzyme levels.