Skip to main content
ARS Home » Southeast Area » Mississippi State, Mississippi » Crop Science Research Laboratory » Genetics and Sustainable Agriculture Research » Research » Publications at this Location » Publication #225674

Title: Interspecific chromosome substitution lines as genetic resources for improvement, trait analysis and genomic inference

item Saha, Sukumar
item Jenkins, Johnie
item McCarty, Jack

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 6/2/2008
Publication Date: 7/1/2008
Citation: Saha, S., Raska, D.A., Jenkins, J.N., McCarty Jr., J.C., Wu, J., Stelly, D.M. 2008. Interspecific chromosome substitution lines as genetic resources for improvement, trait analysis and genomic inference [abstract]. Cotton Science. 20(S):84.

Interpretive Summary:

Technical Abstract: The genetic base that cotton breeders commonly use to improve Upland cultivars is very narrow. The AD-genome species G. barbadense, G. tomentosum, and G. mustelinum are part of the primary germplasm pool, too, and constitute genetic reservoirs of genes for resistance to abiotic stress, pests and pathogens, as well as agronomic and fiber traits. Conventional methods of interspecific introgression into cotton typically entail inbreeding immediately after hybridization or after a few backcrosses. Traditional plant breeding approaches of interspecific crosses have been hindered by many challenges including complex antagonistic relationships between the important traits, genetic drag effect of poor agronomic qualities from alien species, loss of alien genetic materials in early generations, sterility and distorted segregation. An alternative approach to conventional introgression of alien genes for improved traits into an Upland background is via chromosome substitution (CS). Contrary to conventional backcross methods, CS entails the use of hemizygosity to preclude recombination during introgression, so that all genes within a given alien chromosome or chromosome segment are transferred into Upland cotton. We have backcross-substituted (BC5) 20 different chromosomes and chromosome arms of G. barbadense into quasi-isogenic Upland cotton (CS-B) lines. Analyses of CS-B lines based on an additive-dominance genetic model has revealed intriguing effects, including inter-chromosomal epistasis. Plans are to use the CS lines for high-resolution definition and mapping of QTLs associated with important fiber and agronomic traits. Collectively, the CS lines offer an effective and complementary means of germplasm introgression, and the product CS lines are structured well enough to allow for insightful genetic analyses. We expect CS lines for G. tomentosum and G. mustelinum, CS-T and CS- M lines, will be similarly useful.