Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 5/4/2008
Publication Date: 4/1/2009
Citation: Lulai, E.C., Neubauer, J., Thompson, A. 2009. Gene expression Associated with Tuber Periderm Maturation [abstract]. American Journal of Potato Reserach. 86(2):151-152.
Technical Abstract: Potato periderm maturation and associated resistance to tuber excoriation, i.e. skinning injury, is of scientific and agricultural importance because of the losses created by shrinkage, tuber market quality defects and infections. The cells and cellular changes responsible for the development of resistance to excoriation, skin-set, were previously determined. Our current objective is to develop a cadre of candidate genes that may mark skin-set. The periderm is made up of phellem (skin), phellogen (meristematic cell layer or cork cambium) and phelloderm (cortical cells derived from the phellogen). The phellem (skin) is held in place by the phellogen cell walls. As the periderm matures and skin-set develops, the cell walls of the phellogen become stronger and thicker and hold the phellem more tightly in place. Several genes associated with phellogen cell wall modification have been selected as candidates for marker analyses. The genes have been amplified and their identities confirmed after sequencing. Expression profiles of the candidate genes were determined by qRT-PCR during tuber periderm maturation. Wound periderm was used, in part, as a model. The resulting Ct values provide insight into possible use of these genes as markers for periderm maturation and skin-set development. Consistent with past immunolocalization and histological research, expression of pectin methyl esterase genes does not appear to be related to wound periderm development and maturation whereas extensin genes amplify during this period. Genes involved in phellogen activity quickly amplify after wounding. These results are consistent with our hypothesis of the involvement of these genes in wound periderm development and maturation. (poster, physiology, PAA membership #309)