Submitted to: Meeting Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 3/25/2008
Publication Date: 6/1/2008
Citation: Capatana, A., Feng, J., Vick, B.A., Duca, M., Jan, C.C. 2008. Molecular mapping of a new induced gene for nuclear male sterility in sunflower (Helianthus annuus L.). Proceedings of 17th International Sunflower Conference, June 8-12, 2008, Cordoba, Spain. p. 641-644. Interpretive Summary: The male sterility represents the malfunction of plants to produce functional anthers, pollen, or male gametes. It can appear spontaneously or chemically induced via mutations in nuclear and/or cytoplasmic genes. As a result, it leads to the production of a nuclear male sterility (NMS) or genic male sterility (GMS), and cytoplasmic male sterility (CMS), respectively. Nuclear male sterility (NMS) is a valuable breeding tool for producing sunflower hybrid without the tedious emasculation. About 10 Romanian nuclear male sterility lines were established which were under the control of five NMS genes, designated ms1-ms5. Four additional NMS lines were obtained from HA89 treated with mitomycin C and streptomycin. The resulting NMS lines were designed as ms6-ms9. The ms9 gene was mapped to linkage group 10 of the sunflower SSR genetic map. These NMS lines are valuable genetic stocks for hybrid seed production in addition to cytoplasmic male sterility lines. The objectives of current study were to identify molecular markers linked to ms6 in NMS HA89-872, and to map the ms6 locus onto the sunflower linkage group.
Technical Abstract: A new NMS line, NMS HA89-872, induced by mitomycin C and streptomycin carries a single recessive male-sterile gene ms6. An F2 population of 88 plants was obtained from a cross between nuclear male-sterile mutant NMS HA89-872 (msms) and male-fertile line RHA271 (MsMs). 225 SSR primers and 9 RFLP-derived STS primers were selected to screen the polymorphic between male-fertile and male-sterile bulks based on the bulk segregant analysis technique. Nine SSR and three STS markers were polymorphic between two bulks, and used to screen the F2 population. Seven SSR (ORS31, ORS294, ORS495, ORS519, ORS885, ORS807, ORS996) and two STS (STS5C1 and STS11D3) markers were identified to be linked with ms6. The ms6 locus was flanked by ORS 807 and ORS996 at the distance of 7.2 and 18.5 cM, respectively, on the linkage group 16 of sunflower SSR genetic map. The locus order for the SSR and STS markers were similar to the reference maps. These SSR and STS markers had co-dominant and dominant gene actions and would be useful for marker-assisted selection in the breeding programs.