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ARS Home » Southeast Area » Fort Pierce, Florida » U.S. Horticultural Research Laboratory » Subtropical Plant Pathology Research » Research » Publications at this Location » Publication #222160

Title: Identification of three potyviruses in Ammi majus in Florida

item Adkins, Scott
item IREY, M. S. - U. S. SUGAR CORP.
item Rosskopf, Erin

Submitted to: Virus Diseases of Ornamental Plants Symposium Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 2/15/2008
Publication Date: 4/21/2008
Citation: Adkins, S.T., Baker, C., Jones, L., Irey, M., Rosskopf, E.N. 2008. Identification of three potyviruses in Ammi majus in Florida. Virus Diseases of Ornamental Plants Symposium Proceedings.

Interpretive Summary:

Technical Abstract: Virus-like symptoms including mosaic, vein clearing, interveinal chlorosis and rugosity were observed on the leaves of many field-grown Ammi majus plants in the spring 2005 season at several locations in southern Florida. The presence of one or more potyviruses was indicated by inclusion body morphology and testing with a commercially-available enzyme-linked immunosorbent assay (ELISA) for multiple potyvirus species. Sequence analysis of an ~1.6 kb 3’ terminal genome fragment amplified by reverse transcription-polymerase chain reaction (RT-PCR) from total RNA using degenerate potyvirus primers confirmed the diagnosis. The nucleotide and deduced amino acid sequences of the RT-PCR product were 96-97% and 93-99% identical, respectively, to the corresponding region of Clover yellow vein virus (ClYVV) sequences in GenBank. All symptomatic plants tested were infected with potyviruses as determined by ELISA but only a subset was infected with ClYVV suggesting that field symptoms were the result of infection with additional potyviruses. Bidens mottle virus (BiMoV) was subsequently identified. Similar virus-like symptoms were observed in A. majus during the spring 2007 growing season. ELISA testing again indicated the presence of one or more potyviruses. Sequence analysis of RT-PCR products from degenerate potyvirus primers indicated the presence of Apium virus Y (ApVY). A single ApVY-infected sample from mid-season was determined to be co-infected with BiMoV by RT-PCR.