Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: 3/22/2007
Publication Date: 7/30/2007
Citation: Simko, I. 2007. From Association Mapping to Marker-Assisted Selection: Experimental Design and Application. Phytopathology 97:S149.. Interpretive Summary:
Technical Abstract: The most common method for mapping genes in cultivated plant species is genetic linkage mapping. This approach involves generating populations derived from single crosses and estimating the recombination frequencies between marker loci and the genes of interest. However, such mapping populations sample only a small proportion of all possible alleles, which presents a difficulty when developing universal markers for marker-assisted selection. In contrast to genetic linkage mapping, association mapping is a method that detects relationships between phenotypic variation and genetic polymorphisms in existing cultivars, without the need for developing new mapping populations. The method was originally applied in human genetics, but has recently become a useful tool for mapping genes in plants. This linkage disequilibrium-based method effectively incorporates the effect of many past generations of recombination into a single analysis. The association mapping technique can be used in complementation with the linkage mapping method to effectively locate genes segregating in a population of interest. Here I describe an application of the haplotype association mapping technique for detection of molecular markers linked to the Verticillium wilt resistance gene in potato. Discussed will be the main advantages and drawbacks of the association-based mapping methods and a prospect of their application in plant breeding. Examples from autotetraploid potato and diploid lettuce populations will be presented.