|RAVI, K. S.|
|RILEY, D. G.|
|NAIDU, R. A.|
Submitted to: American Phytopathological Society Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 4/9/2007
Publication Date: 6/1/2007
Citation: Ravi, K., Bhanu, P.M., Poojari, S., Kunkalikar, S., Zehr, U., Riley, D., Adkins, S.T., Naidu, R. 2007. Current status of tospoviruses in vegetable cropping systems in India. American Phytopathological Society Annual Meeting.
Technical Abstract: India is the second largest producer of vegetables in the world, accounting for about 15% of global production. Four of the sixteen characterized tospoviruses (genus Tospovirus, family Bunyaviridae) have been reported from the country. Among them, Peanut bud necrosis virus (PBNV), Watermelon bud necrosis virus (WBNV), and Iris yellow spot virus (IYSV) are increasingly becoming major constraints to the production of quality vegetables in different regions of India. We conducted disease surveys to document the prevalence of PBNV, WBNV and IYSV in principal vegetable growing areas. The results indicated wide distribution of PBNV in tomatoes, chili peppers, potatoes and cucurbits, WBNV in watermelon and musk melons, and IYSV in bulb and seed onions. In TAS-ELISA, antibodies raised against PBNV nucleocapsid (N) protein detected both PBNV and WBNV isolates, but not IYSV isolates, in symptomatic plants collected from different regions. In order to establish molecular diversity among PBNV, WBNV and IYSV isolates, N gene sequences were amplified by RT-PCR and the amplicons were cloned and sequenced. A comparison of nucleotide sequences showed more than 94.9 percent identity among forty isolates of PBNV, more than 95.2 percent identity among seventeen isolates of WBNV, and more than 96.3 percent identity among twenty isolates of IYSV.