Submitted to: Biomed Central (BMC) Genomics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/3/2006
Publication Date: 11/3/2006
Citation: Zabala, G., Zou, J., Tuteja, J., Gonzalez, D.O., Clough, S.J., Vodkin, L.O. 2006. Transcription changes in the phenylpropanoid pathway of Glycine max in response to Pseudomonas syringae infection. Biomed Central (BMC) Genomics. 6:26. Interpretive Summary: Paper expands upon our previous microarray project by further examining a set genes involved in production of phenylpropanoid-based chemicals in soybean. These chemicals and the regulation of their synthesis are important in the ability of a plant to adequately defend against pathogens. The results also reveal the opposing demands of different branches of this pathway, one branch leading to production of pigments, the other to production of antimicrobial flavones. Additionally, we detail which of the many different versions of the gene chalcone synthase (key regulated enzyme of this pathway) is most actively involved in defense.
Technical Abstract: Reports of plant molecular responses to pathogenic infections have pinpointed increases in activity of several genes of the phenylpropanoid pathway leading to the synthesis of lignin and flavonoids. The majority of those findings were derived from single gene studies and more recently from several global gene expression analyses. We undertook a global transcriptional analysis focused on the response of genes of the multiple branches of the phenylpropanoid pathway to infection by the Pseudomonas syringae pv. glycinea with or without the avirulence gene avrB to characterize more broadly the contribution of the multiple branches of the pathway to the resistance response in soybean. Transcript abundance in leaves was determined from analysis of soybean cDNA microarray data and hybridizations to RNA blots with specific gene probes.