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ARS Home » Southeast Area » Fort Pierce, Florida » U.S. Horticultural Research Laboratory » Subtropical Plant Pathology Research » Research » Publications at this Location » Publication #201517

Title: Squash vein yellowing virus, a novel ipomovirus, isolated from squash and watermelon in Florida

item Adkins, Scott
item WEBB, S. E.
item ACHOR, D.
item Kousik, Chandrasekar - Shaker

Submitted to: Bemisia International Workshop Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 10/10/2006
Publication Date: 12/1/2006
Citation: Adkins, S.T., Webb, S., Achor, D., Kousik, C.S., Roberts, P.D., Baker, C.A. 2006. Squash vein yellowing virus, a novel ipomovirus, isolated from squash and watermelon in Florida. Bemisia International Workshop Proceedings.

Interpretive Summary:

Technical Abstract: A novel whitefly-transmitted member of the family Potyviridae was isolated from a squash plant (Cucurbita pepo) with vein yellowing symptoms in Florida. The virus, for which the name Squash vein yellowing virus (SqVYV) is proposed, has flexuous rod-shaped particles of ~840 nm in length. SqVYV was transmitted by whiteflies (Bemisia tabaci, biotype B) but was not transmitted by aphids (Myzus persicae). The experimental host range was limited to species in the Cucurbitaceae, with the most dramatic symptoms observed in squash and watermelon, but excluded all tested species in the Amaranthaceae, Apocynaceae, Asteraceae, Chenopodiaceae, Fabaceae, Malvaceae and Solanaceae. Initial greenhouse and field screening of watermelon germplasm with SqVYV has identified potential sources of resistance and experiments are in progress to confirm these preliminary observations. Infection of squash and watermelon by SqVYV induced inclusion bodies visible by electron and light microscopy that were characteristic of members of the family Potyviridae. Comparison of the SqVYV coat protein gene and protein sequences with those of recognized members of the family Potyviridae indicate that it is a novel member of the genus Ipomovirus. A limited survey revealed that SqVYV was present over the five most recent growing seasons in watermelons suffering from a mature vine decline and fruit rot observed in southwest and west central Florida. Inoculation of greenhouse-and field-grown watermelon plants with SqVYV at different stages of growth was sufficient to induce these symptoms, suggesting that it is the likely cause of this disease.