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United States Department of Agriculture

Agricultural Research Service

Title: GENETIC TRANSFORMATION OF SCLEROTINIA SCLEROTIORUM THROUGH AGROBACTERIUM TUMEFACIENS-MEDIATED TRANSFORMATION.)

Author
item Wamatu, John
item White, David
item Chen, Weidong
item Wang, Xin

Submitted to: North American Pulse Improvement Association
Publication Type: Abstract Only
Publication Acceptance Date: 10/1/2005
Publication Date: N/A
Citation:

Interpretive Summary:

Technical Abstract: In order to study genetic factors of pathogenicity, insertional mutants of Sclerotinia sclerotiorum were generated using Agrobacterium tumefacience-mediated transformation with both mycelial fragments and ascospores. Transformants were tested for number of insertions by Southern hybridization and for stability of hygromycin B resistance, and are being screened for altered pathogenicity on lentil plants and for production of oxalic acid. The tagged mutations will allow identification of mutated genes.

Last Modified: 8/24/2016
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