|Kistler, H - Corby|
Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract only
Publication Acceptance Date: 1/14/2006
Publication Date: 1/14/2006
Citation: Boddu, J., Cho, S., Kistler, H.C., Muehlbauer, G. 2006. Deoxynivalenol (DON) influenced gene expression during barley - F. graminearum interaction [abstract]. Plant and Animal Genome XIV Abstracts. p. 282. Interpretive Summary:
Technical Abstract: Fusarium graminearum (teleomorph Gibberella zeae) causes Fusarium head blight (FHB) of barley. Trichothecene mycotoxins, produced by the fungus during infection, play a role in virulence. Loss-of-function mutations in the F. graminearum Tri5 gene, which encodes the first committed enzymatic step in the trichothecene biosynthetic pathway, result in the lack of trichothecene production and reduced virulence. We used the Barley1 and Fusarium Affymetrix GeneChips to examine the genetic mechanisms involved in the host and pathogen during trichothecene accumulation. We isolated RNA from spikes of the barley cultivar Morex inoculated with the Tri5 mutant (non-trichothecene producing) and wild type (trichothecene producing) and hybridized the RNA to the Barley1 and Fusarium GeneChips. We identified 337 barley transcripts that were differentially accumulating in wildtype or Tri5 inoculated plants versus water control inoculated plants. Of these 337 barley transcripts, we detected 123 that were differentially accumulating in plants inoculated with the wildtype strain versus the plants inoculated with the Tri5 mutant strain (P<0.001), indicating that there are barley genes that are up-regulated specifically during trichothecene accumulation. In the same set of 337 barley transcripts, we also detected 26 that were differentially accumulating in plants inoculated with the Tri5 mutant strain compared to plants inoculated with the wildtype strain (P<0.001), indicating that these barley genes may be down-regulated during trichothecene accumulation. Annotations and the functional significance of these differentially accumulating barley transcripts will be presented along with the Fusarium GeneChip results.