|Maroon Lango, Clarissa|
Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: 5/1/2005
Publication Date: 6/30/2005
Citation: Adkins, S.T., Hammond, J., Maroon-Lango, C., Harness, A., Kulemeka, B., Geister, R., Bandla, M., Spiegel, S., Gera, A. 2005. Characterization of a carmovirus from angelonia. Phytopathology. 95:S2.
Technical Abstract: Foliar symptoms and flower break suggestive of virus infection were recently observed on Angelonia angustifolia in several countries. Reverse transcription-polymerase chain reaction products were amplified from total RNA using commercially-available and other degenerate carmovirus group primers. Sequence analysis of these products indicated that this virus was distinct from, but most closely related to, Pelargonium flower break virus (PFBV) and Carnation mottle virus (CarMV), with amino acid identities of 70-76%. However, PFBV and CarMV antisera did not react with symptomatic angelonia tissue by enzyme-linked immunosorbent assay. An agent was mechanically transmitted to Nicotiana benthamiana and virions were isolated from systemically infected leaves, and also directly from angelonia leaves, using a typical carmovirus protocol. Isometric particles ~28 nm in diameter were observed in virion preparations from both hosts, and in thin sections of angelonia floral tissue by electron microscopy. Analysis of dissociated virions by SDS-polyacrylamide gel electrophoresis revealed a major protein component of 33 kDa. Polyclonal antiserum capable of specifically binding virions was produced and will facilitate further characterization, host range studies and surveys. Additional portions of the virus genome are being cloned and sequenced.