Submitted to: Annual Missouri Symposium
Publication Type: Abstract Only
Publication Acceptance Date: 4/7/2005
Publication Date: 4/27/2005
Citation: Ren, C., Chappell, A.S., Beuselinck, P.R., Bilyeu, K.D. 2005. Protein cartography for soybean germination [abstract]. Annual Missouri Symposium. Paper No. 65.
Technical Abstract: To improve soybean seed germination after alterations in seed composition or after exposure to suboptimal conditions, it is necessary to understand the fundamental processes at the molecular level that lead to germination. Soybean seeds are an attractive model for germination because of their large size, lack of dormancy, synchronized germination under permissive conditions, and inability to germinate under cold conditions. The objective of this work was to begin to map and compare the changes in the proteomes of radicles of germinating soybean seeds at permissive and nonpermissive temperatures. Mature dry seeds were imbibed at optimal temperature (25ºC) and inhibiting temperature (4ºC). Proteomes of germinating and non-germinating seed radicles were pairwise compared during different imbibition times by Fluorescence Difference Gel Electrophoresis (DIGE 2-D). Out of more than 1000 detectable protein spots, sixty with significantly different abundances between germinating and non-germinating seeds at each time point were localized by fluorescent coloration. Among the 60 protein spots localized, 30 spots were picked for protein identification by peptide mass fingerprinting and database searching; 15 were putatively identified and 15 were still unknown. Most of the identified proteins associated with storage reserve digestion, but none of them overlapped with the results from proteomic studies of Arabidopsis germination. More comprehensive sets of experiments are ongoing to better fine map the protein changes in the radicle during soybean germination and to determine the identities of proteins related to germination events. We expect to provide a map of the protein events important in the radicle that lead to germination. This map of events may be translated into developing soybean varieties that can germinate under normally nonpermissive conditions or allow germination of seeds with modified compositional qualities.