|VROH, BI IRIE|
|Buckler, Edward - Ed|
Submitted to: Maize Genetics Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 1/5/2005
Publication Date: 3/10/2005
Citation: Briggs, W., Vroh, B., Yamasaki, M., Pressoir, G., Mcmullen, M.D., Gaut, B., Kresovich, S., Buckler Iv, E.S., Doebley, J. 2005. Snp genotyping for diversity and mapping studies in maize and teosinte [abstract]. Maize Genetics Conference. Paper No. 182. p. 129.
Technical Abstract: SNPs are powerful markers for population genetic studies, and linkage and association mapping experiments. SNP have become increasingly available from sequencing data and they are particularly abundant in maize. We designed SNP marker assays within both low-copy EST sequences and known genes for high throughput genotyping in teosintes, landraces, and inbreds. The SNPs were discovered by scanning alignments of EST sequences from 32 diverse maize and teosinte inbreds. Nearly 1000 SNP assays within more than 500 ESTs or genes have been developed to date. A collection of 800 teosinte plants and 278 maize inbreds were genotyped for the markers. Polymorphism indices within the maize inbreds and teosinte as well as the map positions of the markers will be presented. SNP assays including context sequences will be available at www.panzea.org and www.wisc.edu/teosinte.