Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 10/15/2004
Publication Date: 1/15/2005
Citation: St.Amand, P., Guttieri, M.J., Hole, D., Chen, X., Brown Guedira, G.L., Souza, E.J. 2005. Preliminary qtl analysis of dwarf bunt and stripe rust resistance in a winter population [abstract]. Plant and Animal Genome Abstracts.
Technical Abstract: A preliminary genetic map of a recombinant inbred line (RIL) winter wheat (Triticum aestivum L.) population (Rio Blanco/IDO444) was constructed to begin QTL analysis of dwarf bunt (Tilletia controversa) and stripe rust (Puccinia striiformis f. sp. tritici) resistance data. Dwarf bunt disease evaluation was conducted at three locations in Utah and Idaho in 2003 and 2004. RIL stripe rust resistance was evaluated at Mt. Vernon and Pullman, WA in 2003 and 2004. Evaluation of 296 RIL suggests the presence of 5 or more dwarf bunt and 3 or more stripe rust resistance genes. A total of 401 SSR primers amplified scorable fragments, and 159 were scored as polymorphic between the parents. The number of polymorphisms per chromosome ranged from 3 markers each for chromosomes 1A, 1D and 4A to 13 for chromosomes 2D and 6D. No markers on chromosome 6B tested thus far were polymorphic between the parents. All polymorphic markers were evaluated on a subset of 96 RIL. Nineteen linkage groups were formed from the polymorphic markers. QTL analysis identified dwarf bunt resistance linked to DNA fragments on the group 3 chromosomes, including fragments amplified by SSR primers cfd238 and gwm114. Stripe rust resistance was linked in the population to DNA fragments on the group 1 chromosomes amplified by SSR primers cfa2153, barc017, and gwm582. Fragments from the primers gwm148 and barc091 were previously mapped to chromosome 2B, likely on 2BS arm.