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Title: USE OF A MONOCLONAL ANTIBODY AGAINST BABESIA BOVIS MEROZOITE SURFACE ANTIGEN-2C FOR THE DEVELOPMENT OF A COMPETITIVE ELISA TEST.

Author
item DOMINGUEZ, M - INTAC, ARGENTINA
item ZABAL, O - INTAC, ARGENTINA
item WILKOWSKY, S - INTAC, ARGENTINA
item ECHAIDE, I - INTAC, ARGENTINA
item TORIONI, D - INTAC, ARGENTINA
item ASENZO, G - INTAC, ARGENTINA
item RODRIGUEZ, A - INTAC, ARGENTINA
item ZAMORANO, P - INTAC, ARGENTINA
item FARBER, M - INTAC, ARGENTINA
item SUAREZ, CARLOS
item FLORIN-CHRISTENSEN, M - INTAC, ARGENTINA

Submitted to: Annals of the New York Academy of Sciences
Publication Type: Proceedings
Publication Acceptance Date: 8/30/2004
Publication Date: 12/20/2004
Citation: Dominguez, M., Zabal, O., Wilkowsky, S., Echaide, I., Torioni, D.E., Asenzo, G., Rodriguez, A., Zamorano, P., Farber, M., Suarez, C.E., Florin-Christensen, M. 2004. Use of a monoclonal antibody against babesia bovis merozoite surface antigen-2c for the development of a competitive elisa test. Annals Of The New York Academy Of Sciences. 1026:165-170.

Interpretive Summary: In this work we describe the development of a new competitive ELISA assay for the diagnosis of Babesia bovis infection in cattle. The assay is based in a recombinant version of the merozoite surface antigen-2c and a novel monoclonal antibody against it. Preliminary results showed that this test is able to identify specific antibodies against B. bovis from experimentally and naturally infected cattle

Technical Abstract: Bovine babesiosis caused by Babesia bovis is a disease that hampers the production of beef and dairy cattle in tropical and subtropical regions of the world. New diagnostic methods based on recombinant antigens constitute valuable biotechnological tools for the strategic control of this disease. We have developed a competitive enzyme-linked immunosorbent assay that includes a recombinant form of the merozoite surface antigen-2c and a novel monoclonal antibody against it. Preliminary results showed that this test is able to identify specific antibodies against B. bovis from experimentally and naturally infected cattle.