Submitted to: Aflatoxin Elimination Workshop Proceedings
Publication Type: Abstract only
Publication Acceptance Date: 10/1/2004
Publication Date: 12/1/2004
Citation: Lei, Y., Liao, B., Wang, S., Jiang, H., Holbrook, C.C. , Guo, B. 2004. Development of a molecular marker for resistance to seed infection by aspergillus flavus in peanut [abstract]. In: Proceedings of the 2004 Fungal Genomics and Aflatoxin/Fumonisin Elimination Workshop, October 25-28, 2004, Sacramento. p. 64. Interpretive Summary: not required
Technical Abstract: Genetic improvement for host resistance of peanut to fungal colonization and toxin production is an important approach for the integrated management of aflatoxin contamination. Breeding progress has been limited due to the lack of a cost-effective method for identifying resistant individuals in segregating populations. The objective of this research was to attempt to develop a develop a molecular marker that could be used to select for resistance to seed infection by Aspergillus flavus. A resistant (J11) and susceptible (Zhonghua No. 5) parent were selected and used to produce an F2 population. The DNAs of the two parents were extracted and tested with AFLP protocol. From the 256 primer pairs tested, twenty-four pairs showed polymorphism between the two parental lines. The parents and their 108 F2 progeny were tested for reaction to A. flavus infection by inoculation under laboratory conditions. The DNAs of 12 F2 segregating lines extremely resistant and susceptible to seed infection were pooled and analyzed along with individual plants. Two polymorphic markers associated with resistance to seed infection were identified.