Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 1/6/2005
Publication Date: 5/1/2005
Citation: Klasson, K.T. 2005. Spreadsheet tools for determination of derivatives and microbial kinetics (abstract). 27th Symposium on Biotechnology for Fuels and Chemicals. Paper No. 6-55.
Technical Abstract: Determination of enzyme kinetics, microbial growth rates, substrate utilization rates, and product accumulation rates requires derivatives to be calculated with a method that can be duplicated and yield consistent results. Here I discuss two methods that have been suggested in the literature and provide spreadsheet formulas for the needed equations. A cubic spline method proved more versatile in its use even though the spreadsheet programming was elaborate. The methods discussed were tested with published literature data for cell growth and a preferred technique was developed based on smoothing the data and using this smooth data for rate calculations.