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ARS Home » Midwest Area » Urbana, Illinois » Soybean/maize Germplasm, Pathology, and Genetics Research » Research » Publications at this Location » Publication #172621
Title: COMPARING SOYBEAN GENOMIC RESPONSES TO PEST AND PATHOGENS

Author
item Clough, Steven
item ZOU, JIJUN - UNIVERSITY OF ILLINOIS

Submitted to: Cellular and Molecular Biology of Soybean Biennial Conference
Publication Type: Proceedings
Publication Acceptance Date: 6/30/2004
Publication Date: 8/8/2004
Citation: Clough, S.J., Zou, J. Comparing soybean genomic responses to pest and pathogens. Cellular and Molecular Biology of Soybean Biennial Conference. p. 26

Interpretive Summary:

Technical Abstract: Soybean plants are attacked by a wide variety of pests and pathogens requiring effective means of defense. Gene activation is a major mechanism to reduce the degree of damage caused by pests and pathogen invasion as it leads to the rapid, regulated production of toxins and enzymes in addition to structural reinforcements. We used soybean cDNA microarrays to measure differential gene expression in response to soybean aphid Aphis glycines and against the causal agents of three different diseases. Pseudomonas syringae, with or without avrB, allowed for the comparison of the hypersensitive defense response to susceptibility. Inoculating differential lines with Sclerotinia sclerotiorum allowed the study of quantitative resistance in stem infections. Treatment of fresh cuttings with sterile culture filtrate allowed the study of soybean response to mycotoxin released by Fusarium solani. Expression similarities and differences between these soybean-pest/pathogen interactions will be discussed.