Submitted to: Soybean Biotechnology Meeting
Publication Type: Proceedings
Publication Acceptance Date: 6/30/2004
Publication Date: 8/8/2004
Citation: Zou, J., Lee, J., Xu, S.S., Cregan, P.B., Clough, S.J., Hymowitz, T. 2004. Assignment of molecular linkage groups to soybean chromosomes. Soybean Biotechnology Meeting. p.12.
Technical Abstract: Singh and Hymowitz (1988) published the first soybean chromosome map based on pachytene chromosome analysis. This laid the foundation to identify primary trisomics in the soybean (Xu et al. 2000). We have assigned 11 molecular linkage groups to soybean [Glycine max (L.) Merr., 2n = 40] chromosomes by using primary trisomics (2x + 1 = 41) and SSR markers. Primary trisomics were hybridized with G. soja Sieb and Zucc. (2n = 40) in the greenhouse, F1 plants with 2n = 40 and 41 were identified cytologically and 41 chromosome plants were selfed. A deviation from 1:2:1 ratio in the F2 population suggests a marker is associated with a chromosome. The relationships between soybean chromosomes and molecular linkage groups are: 1 = D1a+q, 3 = N, 5 = A1, 8 = A2, 9 = K, 13 = F, 14 = C1, 17 = D2, 18 = G, 19 = L and 20 = I. Updated information on gene expression profiling (ca. 10,000 genes) using primary trisomic and tetrasomic (2x + 2 = 42) to study single and double chromosomes effects will be presented.