Submitted to: Soybean Biotechnology Meeting
Publication Type: Proceedings
Publication Acceptance Date: 6/30/2004
Publication Date: 8/8/2004
Citation: Zou, J., Rodriguez-Zas, S., Gonzales, D., Vodkin, L., Clough, S.J. 2004. Expression profiling of soybean responses to bacterial pathogen pseudomonas syringae. Soybean Biotechnology Meeting. p. 114.
Technical Abstract: The interactions between pathogens and a plant host are often classified as being either compatible or incompatible. Under compatible interactions, the virulent (VIR) pathogen succeeds in causing disease, whereas in incompatible interactions, host resistance is quickly established, accompanied by a hypersensitive response (HR) leading to cell death of plant cells in intimate contact with the pathogen. Genomic responses that occur under both VIR and HR interactions in soybean were determined by mRNA expression profiling using cDNA microarrays representing over 27,000 different soybean sequence tags. Soybean leaves were infiltrated with Pseudomonas syringae pv. glycinea with or without avrB, or 10 mM MgCl2 as an inoculation control. The replicated experiment was statistically analyzed by ANOVA to identify about 4,000 genes undergoing significant changes in transcript abundance in different time points. Microarray results of selected genes were validated with quantitative real-time RT-PCR. The unique expression patterns for HR and VIR and cross comparisons of current results with soybean responses to other pathogens will be presented.