Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 11/20/2004
Publication Date: 9/1/2005
Citation: Weiland, J.J., Shelver, W.L. 2005. Antiserum for the detection of aphanomyces cochlioides [Abstract]. Journal of Sugar Beet Research. 42(1&):60.
Technical Abstract: Quantitative immunological and nucleic acid detection methods for Aphanomyces have proven useful for the breeding of leguminous crops. A program objective is to develop quantitative tools for the detection of Aphanomyces cochlioides in sugarbeet fields and in inoculated, greenhouse-grown sugarbeet. A cell-wall preparation of A. cochlioides was used to immunize New Zealand white rabbits in an effort to raise antisera to this organism. Antiserum from rabbit 114 reacted significantly better with Aphanomyces spp than with any other common pathogen of sugarbeet tested, generating strong positive signals in a direct enzyme linked immunosorbant assay (ELISA) within 30 min. of substrate addition. Due to inherent low reactivity with components in healthy sugarbeet extracts, the antiserum was capable of discriminating sugarbeet seedlings infected with A. cochlioides from uninfected seedlings. Use of the antiserum demonstrated that sugarbeet roots received at piling stations in the Red River Valley of Minnesota, although exhibiting characteristic Aphanomyces chronic phase symptoms, possessed little reactive material.