|LA ROTA, C|
Submitted to: Wheat Genetics International Symposium Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 6/30/2003
Publication Date: 9/1/2003
Citation: Linkiewicz, A.M., Qi, L., Echalier, B., Gill, B.S., Chao, S., Lazo, G.R., Anderson, O.D., Akhunov, E.D., Dvorak, J., Miftahudin, Gustafson, J.P., La Rota, C.M., Sorrells, M.E., Hossain, K., Kalavacharla, V., Kianian, S.F., Dilbirligi, M., Gill, K.S., Zhang, D., Nguyen, H.T., Wennerlind, E., Peng, J., Lapitan, N., Anderson, J.A., Fenton, R.D., Close, T.J., Mcguire, P.E., Qualset, C.O., Dubcovsky, J. 2003. A two-thousand loci physical map of wheat homoeologous group 5. In: Proceedings of the Wheat Genetics International Symposium, September 1-6, 2003, Paestum, Italy. p. 986-988.
Technical Abstract: As part of the NSF-funded Wheat Expressed Sequence Tag (EST) Project, we mapped 7,000 unigenes in a set of 102 deletion lines (http://wheat.pw.usda.gov/NSF/). We summarize here the results for homoeologous group 5. A total of 2,338 loci were mapped with 1,052 EST probes and used to construct a consensus map including 28 bins. A relatively higher number of ESTs were mapped on the B genome (38%) compared to the A (34%) and D (29%) genomes. The B genome also showed a higher level of duplicated loci (42%). Differences in the level of polymorphism were only partially responsible for these differences. The long arms showed three times more probes than the short arms, and in both arms, the distal halves showed a significantly higher number of probes, loci, and duplicated loci than the proximal halves. A good overall colinearity was observed among the three genomes, except for the previously known 5AL/4AL translocation and a new small pericentric inversion in chromosome 5A. Statistically significant colinearity was observed between wheat homoeologous group 5 and rice chromosomes 12 (88 ESTs), 9 (72 ESTs), and 3 (84 ESTs). This colinearity can be used to infer the order of the wheat ESTs within the bins.