|Srinivasan, K. g.|
|Min, B. e.|
|Ryu, K. h.|
|Wong, S. m.|
Submitted to: Archives of Virology
Publication Type: Peer reviewed journal
Publication Acceptance Date: 7/5/2004
Publication Date: 10/5/2004
Citation: Srinivasan, K. G., Min, B. E., Ryu, K. H., Adkins, Scott, Wong, S. M. 2004. Complete Sequence of Hibiscus Latent Singapore Virus Containing an Internal Poly(A) Tract and Ires Elements. Archives of Virology. Interpretive Summary: Tobamoviruses are studied extensively and they have been useful tools for understanding the fundamental processes of virus infection, replication and movement. The virus genus consists of 19 definitive species that can be classified into three sub-groups based on their host range and genome organization. Complete sequences of several tobamoviruses have been reported. Earlier we reported the discovery and partial nucleotide sequence of a recently discovered hibiscus-infecting tobamovirus, Hibiscus latent Singapore virus (HLSV). Here we report the host range, full-length sequence of HLSV, a comparison to other tobamoviruses and unique genome features.
Technical Abstract: We have completed sequencing the genome of a hibiscus-infecting tobamovirus, Hibiscus latent Singapore virus (HLSV). The genomic structure of HLSV is similar to other tobamoviruses. It consists of a 5' untranslated region (UTR), followed by ORFs encoding for a 128 kDa protein and a 186 kDa readthrough protein, movement protein (MP), coat protein (CP) and a 3' UTR. The unique feature of HLSV is the presence of a poly(A) tract within its 3' UTR. This internal poly(A) tract which varies in length was found immediately downstream of the CP stop codon. Studies directed towards understanding the translational strategies involved in expression of 3' proximal genes in HLSV led to the identification of two putative IRESs that were located upstream of MP and CP ORFs. We report here the analysis of HLSV genomic sequence and propose that HLSV is a new tobamovirus.