|HANSON, S. F.|
Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: 5/1/2004
Publication Date: 6/1/2004
Citation: Allen, J., Kamenova, I., Adkins, S.T., Hanson, S. 2004. Detection of hibiscus latent Fort Pierce virus in New Mexico. Phytopathology. 94:S4.
Technical Abstract: Hibiscus latent Fort Pierce virus (HLFPV) is a newly described tobamovirus recently isolated from landscape plantings of Hibiscus rosa-sinensis in Florida. Consistent with members of the tobamovirus group, HLFPV is mechanically transmitted and causes economic losses by damaging the leaves and flowers of the host. Because hibiscus is a popular ornamental planted nationwide, we sought to determine if HLFPV was present in areas outside of Florida. Dot blot immunoassay (DBIA) and tissue blot immunoassay (TBIA) were used to identify potentially infected plants in New Mexico. Electron microscopy showed the presence of tobamovirus-like rods in immuno-positive samples. Extracts from infected and uninfected tissue were used in immunocapture-reverse-transcription-polymerase chain reaction (IC-RT-PCR) using primers specific for the HLFPV coat protein ORF. PCR products were detected only in immuno-positive samples. Sequence analysis showed that the PCR products were 100% identical to HLFPV indicating that this virus is present in New Mexico.