Submitted to: International Conference on Legume Genomics and Genetics
Publication Type: Abstract Only
Publication Acceptance Date: 6/7/2004
Publication Date: 7/10/2004
Citation: Clough, S.J., Zou, J., Vuong, T., Li, M., Li, Y., Hartman, G.L., Rodriguez-Zas, S., Vodkin, L.O. 2004. Expression profiling of soybean response to pests and pathogens [abstract]. International Conference on Legume Genomics and Genetics. p. 246.
Technical Abstract: Soybean plants are attacked by a wide variety of pests and pathogens requiring effective means of defense. Gene activation is a major mechanism to reduce the degree of damage caused by pests and pathogen invasion as it leads to the rapid, regulated production of toxins and enzymes in addition to structural reinforcements. We used soybean cDNA microarrays to measure differential gene expression in response to soybean aphid (Aphis glycines) and 3 different pathogens. The plant-pathogen interactions that were analyzed in this study included the Pseudomonas syringae induced hypersensitive response versus virulence on leaves, quantitative resistance to Sclerotinia sclerotiorum infection in stem tissue, and the effect of Fusarium solani toxin on leaf tissue in highly sensitive (Essex) and partially resistant (Williams and PI567.374) cultivars. ANOVA analysis of expression data from tens of thousands of spotted gene representatives identified hundreds of genes that were significantly activated or repressed during these interactions. Summaries of the results of these soybean-pest/pathogen interactions will be presented.