|Lockhart, Benham, e.|
Submitted to: Plant Health Progress
Publication Type: Peer reviewed journal
Publication Acceptance Date: 7/14/2004
Publication Date: 8/9/2004
Citation: Timur, M.M., Lockhart, B.L., Dankers, H., Adkins, S.T. 2004. Canna yellow mottle virus detected in canna in Florida. Plant Health Progress doi:10.1094/PHP-2004-0809-01-HN. Interpretive Summary: This is the first report of canna yellow mottle virus (CaYMV) infecting canna in Florida. A description of the symptoms and report of incidence are included. Diagnostic methods used to confirm the identity of CaYMV are also described. This report continues a cooperative virology research effort between ARS and University of Florida and extends it to include University of Minnesota. It also provides a timely account of CaYMV infection of canna to growers, Extension personnel and state and Federal regulatory and research scientists.
Technical Abstract: Symptoms similar to canna yellow mottle disease caused by canna yellow mottle virus (CaYMV), a badnavirus, were observed on outdoor and greenhouse grown canna plants in Florida, in spring 2003 and January 2004, respectively. Symptoms observed included foliar chlorotic and necrotic mottle and veinal streaking. The incidence of symptomatic canna was more than 60% in the affected areas in both years. Leaf samples of symptomatic plants were examined by electron microscopy (EM) and by immunosorbent electron microscopy (ISEM) using partially purified extracts and a broad-spectrum antiserum to related viruses. Typical badnavirus-like particles were observed in extracts from symptomatic but not from asymptomatic canna plants. The presence of CaYMV in symptomatic plants was confirmed by PCR amplification using CaYMV- specific primers. No PCR product was obtained from total DNA similarly extracted from asymptomatic plants. Based on symptoms, EM, ISEM, and PCR it is concluded that CaYMV is present in Florida. Canna yellow mottle can be an economically important disease constraint to the production of cannas in the southeastern US.