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ARS Home » Plains Area » Fargo, North Dakota » Edward T. Schafer Agricultural Research Center » Food Animal Metabolism Research » Research » Publications at this Location » Publication #162461


item Shappell, Nancy
item Billey, Lloyd

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 4/3/2004
Publication Date: 7/25/2004
Citation: Shappell, N.W., Billey, L.O. 2004. Assessment of tasco and ycwp on ergovaline toxicity in caco-2 cells. [abstract] Joint Meeting of American Society of Animal Science, American Dairy Science Association, and Poultry Science Association, St. Louis, MO, July 25-29, 2004, Journal of Animal Science 82(Suppl 1):181, Abstract T72.

Interpretive Summary:

Technical Abstract: The seaweed extract Tasco and yeast cell wall preparation (YCWP) were tested to evaluate efficacy in attenuation of ergovaline toxicity to Caco-2 cells. Initially cells were treated with 0.1% to 0.001% Tasco to identify potential Tasco toxicity. By ~24h at 37º C in the presence of media (with or without cells) globules formed. After consultation with manufacturers, it was concluded that calcium and magnesium concentrations present in the medium were causing coalescence of the alginates present in Tasco resulting in globules. Tasco was toxic at high concentrations (72h, 0.001% to 0.1% yielded ~66% to 1% of control values, respectively) as assessed by metabolic activity (using the alamarBlue assay) and total protein. Ergovaline's toxicity (100 µM) was tested +/- Tasco (0.0001% and 0.00005%, 72h) on undifferentiated cells. At these concentrations Tasco was non-toxic and it did not alter the toxicity of ergovaline (~40% reduction of metabolic activity and/or total protein relative to control cells with ergovaline treatment). As YCWP is not water soluble, DMSO "extraction" was used (0.1g/ml). The cellular effect of YCWP in DMSO (ranging from 0.1-0.5%) was evaluated on cells. DMSO alone was found to reduce metabolic activity and total protein at concentrations greater than 0.2%. Therefore, YCWP was tested in 0.05% and 0.1% DMSO. At the 0.05% DMSO/YCWP there was a slight amelioration of the toxicity caused by ergovaline (10-20%). At the 0.1% concentration no amelioration was seen, and ergovaline toxicity may have been increased slightly (<10%). Further investigation of Tasco and YCWP at different concentrations may be warranted. An unexpected finding was the reduction of ergovaline toxicity when cells were treated in the presence of DMSO.